科研之友
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摘要
AIM: To study the actions of sodium suberogorgin (Sub) on normal- and myocardial ischemic-reperfused-working hearts. METHODS: The isolated guinea pig working hearts were perfused with edetic acid (0.2 mu mol . L(-1))-Krebs-Henseleit solution by left pulmonary vein at 37 +/- 0.5 degrees C. The perfusing pressure of left atrium and the afterload of left ventricle were maintained at 1.0 and 7.2 kPa, respectively. During 50-min low-flow global ischemia, coronary flow was maintained at 4.7 +/- 0.2 % of normal working hearts via retrograding perfusion. Reperfusion lasted 35 min following ischemia. RESULTS: Before ischemia, the aortic pressure, left ventricular systolic pressure, the maximal rate of left ventricular pressure change (+ dp/dt(max) and - dp/dt(max)), cardiac output, stroke volume, left ventricular end-diastolic pressure, and heart rate of working hearts treated with Sub 10 mu mol . L(-1) for 10 min were increased by 14 %, 17 %, 17 %, 22 %, 15 %, 32 %, - 200 %, and - 11 %, respectively. These parameters were reduced when Sub was increased to 50 mu mol . L(-1). Reperfusion aggravated the myocardial damages induced by ischemia. Sub 10 mu mol . L(-1) used before and during low-flow ischemia completely restored all above parameters except heart rate to the level of preischemia. When Sub was increased to 50 mu mol . L(-1), only an attenuation of myocardial damage was observed. CONCLUSION: Sub obviously improved the ischemic-reperfused injury in isolated working hearts.
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