Novel magnetic and fluorescent nanocomposite as a sensitive probe for the determination of proteins

作者：Wang Lun; Hong Shi; Wang Leyu; Dong Li; Bian Guirong; Xia Tingting; Chen Hongqi

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2006, 65(2): 439-444.

DOI：10.1016/j.saa.2005.11.023

• magnetic nanocomposites
• Fe3O4/(NaOL/CTAB)(n)/PABA
• fluorescence probe
• self-assembly approach

The Fe3O4/(sodium oleic acid/ethyltrimethyl ammonium bromide)(n)/4-aminobenzoic acid (Fe3O4/(NaOL/CTAB)(n)/PABA) nanocomposites have been prepared by a layer-by-layer self-assembly approach. This kind of nanocomposites have fluorescent, magnetic and water-soluble properties. Taking advantage of the magnetic property of nanocomposites, we can separated them from solution easily by using a permanent magnet. By using their strong fluorescence, we can detect proteins. At pH 6.98, the fluorescence of Fe3O4/(NaOL/CTAB)(n)/PABA nanocomposites can be enhanced by the proteins. Under optimal conditions, the linear ranges of calibration curves were 0.2-20, 0.2-13, 0.2-10 mu g mL(-1) for gamma-globulin (gamma-IgG), human serum albumin (HSA), and bovine serum albumin (BSA), respectively. The detection limits were 0.02, 0.01, 0.02 for gamma-IgG, HSA and BSA, respectively. The method has been applied to analyze the total proteins in human samples and the results were in good agreement with those reported by the hospital. This method is sensitive, simple and potential in many areas.

• 出版日期2006-10
• 单位安徽师范大学