科研之友
微信
新浪微博
Facebook
分享链接
摘要
A novel Nano-Fe3O4 particles biosensor for glucose based on luminol electrochemiluminescence (ECL) is presented. Glucose oxidase (GOD) was covalently cross-linked to the surface of synthesized magnetic nano-Fe3O4 particles. Then the composite particles Fe3O4/GOD was adhered to solid paraffin carbon paste electrode by magnetic force to fabricate a working electrode. Hydrogen peroxide was produced by enzymatic reaction of GOD and ECL could be obtained by the reaction between luminol and hydrogen peroxide to construct the renewable glucose biosensor. There was a linear relationship between ECL and glucose concentration in the ranges of 1 x 10(-5) - 1. 0 x 10(-2) mol/L with a regression equation of I = 65. 4374C + 23. 9017 (r = 0. 9987), and the detection limit is 1 mu mol/L. The ECL biosensor has showed short response time and high stability, and the electrode surface was renewable easily. The proposed biosensor has been applied to the determination of glucose in plasma samples.
- 出版日期2010-6
- 单位桂林理工大学
