Dynamic dual-tracer MRI-guided fluorescence tomography to quantify receptor density in vivo

作者：Davis Scott C^*; Samkoe Kimberley S; Tichauer Kenneth M; Sexton Kristian J; Gunn Jason R; Deharvengt Sophie J; Hasan Tayyaba; Pogue Brian W

来源：Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(22): 9025-9030.

DOI：10.1073/pnas.1213490110

• molecular imaging
• oncology
• optical imaging
• engineered proteins
• spectroscopy

The up-regulation of cell surface receptors has become a central focus in personalized cancer treatment; however, because of the complex nature of contrast agent pharmacokinetics in tumor tissue, methods to quantify receptor binding in vivo remain elusive. Here, we present a dual-tracer optical technique for noninvasive estimation of specific receptor binding in cancer. A multispectral MRI-coupled fluorescence molecular tomography system was used to image the uptake kinetics of two fluorescent tracers injected simultaneously, one tracer targeted to the receptor of interest and the other tracer a nontargeted reference. These dynamic tracer data were then fit to a dual-tracer compartmental model to estimate the density of receptors available for binding in the tissue. Applying this approach to mice with deep-seated gliomas that overexpress the EGF receptor produced an estimate of available receptor density of 2.3 +/- 0.5 nM (n = 5), consistent with values estimated in comparative invasive imaging and ex vivo studies.

• 出版日期2013-5-28