Studies on the prevalence of Babesia canis canis in marsh ticks (Dermacentor reticulatus) in the Saarland

作者：Beelitz Pamela^*; Schumacher Stefan; Marholdt Fritz; Pfister Kurt; Silaghi Cornelia

来源：Berliner und Munchener Tierarztliche Wochenschrift, 2012, 125(3-4): 168-171.

DOI：10.2376/0005-9366-125-168

• Dermacentor reticulatus
• prevalence
• Babesia canis canis
• Southwest Germany

An accumulation of autochthonous cases of canine babesiosis caused by Babesia canis has been registered in a small animal clinic in the Saarland since the beginning of 2006, some cases with fatal outcome. This study aims to contribute to the explanation of strong focal occurrence of infections with B. canis in this region. Therefore, patient owners who had presented their dogs in the years 2006 and 2007 because of babesiosis and who had claimed not having left the Saarland with their dogs at least six months before the outbreak of Babesiosis, were asked for their dog walking habits. Accordingly, a selection of ten tick collection sites of various landscape structures was made. Tick sampling by flagging the vegetation took place every month from March to December 2008. The collected ticks were identified morphologically. In eight of ten collecting sites a total of 397 adult Dermacentor reticulatus ticks were collected from March to December with the highest frequencies during the months of May, October and November. All collected specimens were examined by genus-specific conventional PCR for the presence of Babesia-DNA. In positive samples, the PCR-products were differentiated by sequencing. ten D. reticulatus (2.5%) ticks examined were found positive for DNA of B. canis canis originating from three out of eight collection sites. Consequently, an endemic distribution of D. reticulatus was confirmed and a natural focus of B. canis canis was detected in the area southwest of Saarbrucken.

• 出版日期2012-4