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摘要
X-ray phase contrast imaging (PCI) provides excellent image contrast by utilizing the phase shift. The introduction of microbubbles into tissues can cause a phase shift to make microbubbles visibly identified on PCI. In this study, we assessed the feasibility of targeted microbubble-based PCI for the detection of thrombosis. @@@ The absorption and phase contrast images of P-selectin-targeted microbubbles (MBP) were obtained and compared in vitro. MBP, control IgG-targeted microbubbles (MBC), and unbound microbubbles (MBU) were tested for binding specificity on thrombi expressing P-selectin. MBP were used as molecular PCI probes to evaluate P-selectin expression in a mouse model of arteriovenous shunt thrombosis that was created using PE tubes in the bypass outside of the mouse body. @@@ PCI clearly showed the microbubbles not viewable via absorption contrast imaging (ACI). In vitro attachment of MBP (91.60 +/- 11.63) to thrombi was significantly higher than attachment of MBC (17.80 +/- 4.02, P < 0.001) or MBU (9.80 +/- 2.59, P < 0.001). In the mouse model of arteriovenous shunt thrombosis, the binding affinity of MBP (15.50 +/- 6.25) was significantly greater than that of MBC (0.50 +/- 0.84, P < 0.001) or MBU (0.33 +/- 0.52, P < 0.001). @@@ Our results indicate that molecular PCI may be considered as a novel and promising imaging modality for the investigation of thrombosis. @@@ aEuro cent Small thrombi are rarely detected by conventional radiography. @@@ aEuro cent Phase contrast imaging (PCI) provides higher contrast and spatial resolution than conventional radiography. @@@ aEuro cent P-selectin targeted microbubbles detected by PCI may suggest early thrombosis.
- 出版日期2016-9
- 单位上海交通大学; 上海市闵行区中心医院
