Comparison of real-time reverse-transcription loop-mediated isothermal amplification and real-time reverse-transcription polymerase chain reaction for detection of noroviruses in municipal wastewater

作者：Suzuki Yoshihiro^*; Narimatsu Shogo; Furukawa Takashi; Iwakiri Akira; Miura Miho; Yamamoto Shogo; Katayama Hiroyuki

来源：Journal of Bioscience and Bioengineering, 2011, 112(4): 369-372.

DOI：10.1016/j.jbiosc.2011.06.012

• Noroviruses
• Real-time RT-LAMP
• Real-time RT-PCR
• Monitoring
• Wastewater
• Sensitivity

The monitoring of NVs in municipal wastewater by both real-time RT-IAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1<p) for both NV-GI and NV-GII.

• 出版日期2011-10