Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice

作者：Wang K W; Chueh L L; Wang M H; Huang Y T; Fang B H; Chang C Y; Fang M C; Chou J Y; Hsieh S C; Wan C H^*

来源：Laboratory Animals, 2013, 47(2): 116-121.

DOI：10.1177/0023677213478298

• Minute virus of mice
• mouse parvovirus
• alpha-actin
• multiplex PCR

Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (alpha-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.

• 出版日期2013-4