Antiserum preparation against Rice dwarf virus Kunming isolate and detection by immunocapture PCR tests

作者：Peng L B; Li T T; Wei C H; Fang Q; Dong J H; Zhang L Z; Su X X; Ding M; Zhang Z K^*; Li Y

来源：Virologica Sinica, 2006, 21(4): 408-410.

• Antiserum
• I-ELISA
• IC-PCR
• Rice dwarf virus (RDV)

By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA. Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.

• 出版日期2006
• 单位北京大学