A new selective chromogenic and turn-on fluorogenic probe for copper(II) in solution and vero cells: recognition of sulphide by [CuL]

作者：Mahapatra Ajit Kumar^*; Mondal Sanchita; Manna Saikat Kumar; Maiti Kalipada; Maji Rajkishor; Uddin Md Raihan; Mandal Sukhendu; Sarkar Deblina; Mondal Tapan Kumar; Maiti Dilip Kumar

来源：Dalton Transactions, 2015, 44(14): 6490-6501.

DOI：10.1039/c4dt03969f

A new coumarin-appended thioimidazole-linked imine conjugate, viz. L has been synthesized and characterized. L has been found to recognize Cu2+ selectively among a wide range of biologically relevant metal ions. The chemosensing behavior of L has been demonstrated through fluorescence, absorption, visual fluorescence color changes, ESI-MS and H-1 NMR titrations. The chemosensor L showed selectivity toward Cu2+ by switch on fluorescence among the 18 metal ions studied with a detection limit of 1.53 mu M. The complex formed between L and Cu2+ is found to be 1 : 1 on the basis of absorption and fluorescence titrations and was confirmed by ESI-MS. DFT and TDDFT calculations were performed in order to demonstrate the structure of L and [CuL] and the electronic properties of chemosensor L and its copper complex. This highly fluorescent [CuL] complex has been used to recognize sulphide selectively among the other allied anions. Microstructural features of L and its Cu2+ complex have been investigated by SEM imaging (scanning electron microscopy). The biological applications of L were evaluated in Vero cells and it was found to exhibit low cytotoxicity and good membrane permeability for the detection of Cu2+.

• 出版日期2015