Alginate-encapsulation, short-term storage and plant regeneration from protocorm-like bodies of Aranda Wan Chark Kuan %26apos;Blue%26apos; x Vanda coerulea Grifft. ex. Lindl. (Orchidaceae)

作者：Gantait Saikat; Bustam Suryanti; Sinniah Uma Rani^*

来源：Plant Growth Regulation, 2012, 68(2): 303-311.

DOI：10.1007/s10725-012-9699-x

• Acclimatization
• Conversion
• Germplasm conservation
• Germplasm exchange
• PLB
• Synthetic seeds

This article demonstrates the single bead alginate-encapsulation and conversion (complete plantlet regeneration) from protocorm-like bodies (PLBs) of Aranda Wan Chark Kuan %26apos;Blue%26apos; x Vanda coerulea Grifft. ex. Lindl. (AV) (a monopodial orchid hybrid) for the first time. PLBs, induced from leaf segments of AV were isolated from in vitro proliferating PLB clusters. Individual PLBs (4 +/- A 1 mm diameter) were encapsulated in calcium alginate beads to manage mass propagation, short-term storage and germplasm sharing. The superior gel matrix for encapsulation was obtained using 3 % sodium alginate and 75 mM calcium chloride (CaCl2 center dot 2H(2)O). Highest percentage of germination (98.1 %) and conversion (96.2 %) of encapsulated PLBs (capsules) was obtained on plant growth regulator-free half-strength MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) medium. Successful storage of capsules, until 180 days, was achieved at 25 A degrees C under zero-irradiance with germination and conversion frequency of 76.9 and 70.2 %, respectively. Plantlets regenerated from capsules were acclimatized successfully with 92 % survival rate.

• 出版日期2012-11