A 4-Week Repeated Oral Dose Toxicity Study of Dairy Fat Naturally Enriched in Vaccenic, Rumenic and alpha-Linolenic Acids in Rats

作者：Anadon Arturo; Rosa Martinez Larranaga Maria; Aranzazu Martinez Maria; Ares Irma; Ramos Eva; Gomez Cortes Pilar; Juarez Manuela; Angel de la Fuente Miguel

来源：Journal of Agricultural and Food Chemistry, 2011, 59(14): 8036-8046.

DOI：10.1021/jf201251g

• dairy fat
• vaccenic acid
• rumenic acid
• alpha-linolenic acid
• safety evaluation
• triglyceridemia

Few studies have focused on the toxicological risks of dairy fat intake. A standard dairy fat (SDF) with a 70% SPA content and a naturally enriched dairy fat (EDF) in vaccenic, rumenic and a-linolenic acids and low in SPA (54%) have been examined in a 4-week repeated dose oral toxicity study as a daily dose of 2000 mg/kg bw by gavage in rats. Comparisons were established with a third group of rats (control) which did not receive fat administration. Both fats were well tolerated, and no adverse events or mortality were observed during the treatment nor after a 2-week observation period. EDF and SDF did not cause significant differences with respect to a control group in body weight gain, food consumption, clinical observations, organ weight ratios, histopathological findings and most of the hematological and biochemical parameters including total cholesterol and cholesterol fractions in plasma. In rats treated with SDF, a significant increase of triglycerides was observed as compared to the control group. By contrast, in rats treated with EDF, a significant decrease in triglycerides was detected. EDF orally administered to rats was safe, and no treatment-related toxicity was detected. The results also suggest that EDF could protect against the increase of triglyceride concentrations in plasma.

• 出版日期2011-7-27