B23 acts as a nucleolar stress sensor and promotes cell survival through its dynamic interaction with hnRNPU and hnRNPA1

作者：Yao Z; Duan S; Hou D; Wang W; Wang G; Liu Y; Wen L; Wu M^*

来源：Oncogene, 2010, 29(12): 1821-1834.

DOI：10.1038/onc.2009.473

• B23/NPM
• hnRNPs
• nucleolar stress
• cell survival
• apoptosis

The nucleolus is one of the functional nuclear compartments in which the ribosome biogenesis takes place. Proteomic analysis revealed a large number of nucleolar proteins that are involved in diverse cellular processes, including biogenesis of ribonucleoprotein particles, stress responses, cell proliferation and cell cycle progression. Here, we report that in response to transcription repression-induced nucleolar segregation, B23, a nucleolar protein required for rRNA processing, is localized to the cytoplasm and forms a complex with the mRNA-binding proteins hnRNPU and hnRNPA1 in a sequential manner. In addition, RNA Polymerase I, but not RNA Polymerase II inhibition, was found to account for the translocation-dependent assembly of B23/hnRNPU/hnRNPA1. We also showed that interactions among these proteins are regulated by hnRNPU-bound mRNAs such as the 30-untranslated region (UTR) of Bcl-xL mRNA. Ectopically expressed 30-UTR of Bcl-xL mRNA, which disrupted the interactions among B23, hnRNPU and hnRNPA1, led to enhanced cell apoptosis induced by either actinomycin D treatment or mitotic arrest. Overall, these data highlight a novel function of B23 as a stress sensor in the assembly of B23/hnRNPU/hnRNPA1 complex to promote cell survival. Oncogene (2010) 29, 1821-1834; doi:10.1038/onc.2009.473; published online 18 January 2010

• 出版日期2010-3
• 单位中国科学技术大学