Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein

作者：Jalilian Farid Azizi; Jahanshiri Fatemeh; Sekawi Zamberi; Omar Abdul Rahman; Yusoff Khatijah^*

来源：Biotechnology and Applied Biochemistry, 2011, 58(2): 97-102.

DOI：10.1002/bab.19

• protein expression
• respiratory syncytial virus
• G immunogenic domain

Despite all efforts, there is still no effective vaccine available against the human respiratory syncytial virus (HRSV) that is a major cause of severe lower respiratory tract disease in infants and the elderly. In this review, we examined the potential of the conserved immunogenic region (residues 122-226) of the HRSV glycoprotein G alone as the inducer of neutralizing antibodies against this virus. The Escherichia coli produced recombinant conserved region of G (designated as G domain), which was used for rabbit immunization. Although rabbit is semipermissive host for HRSV, our result showed that the polyclonal antibodies against the G domain protein could strongly neutralize the virus (69.3%), suggesting that the G immunogenic region of HRSV alone has a great potential in vaccine development. To our knowledge, this is the first report in which neutralizing antibodies to respiratory syncytial virus have been evoked using bacterially expressed G immunogenic domain protein without any adjuvant.

• 出版日期2011-4