ERIS, an endoplasmic reticulum IFN stimulator, activates innate immune signaling through dimerization

作者：Sun, Wenxiang; Li, Yang; Chen, Lu; Chen, Huihui; You, Fuping; Zhou, Xiang; Zhou, Yi; Zhai, Zhonghe; Chen, Danying; Jiang, Zhengfan^*

来源：Proceedings of the National Academy of Sciences, 2009, 106(21): 8653-8658.

DOI：10.1073/pnas.0900850106

• innate immunity
• type I IFN
• functional cDNA library screening
• cytosolic RNA and dsDNA
• ER retention signal

We report here the identification and characterization of a protein, ERIS, an endoplasmic reticulum (ER) IFN stimulator, which is a strong type I IFN stimulator and plays a pivotal role in response to both non-self-cytosolic RNA and dsDNA. ERIS (also known as STING or MITA) resided exclusively on ER membrane. The ER retention/retrieval sequence RIR was found to be critical to retain the protein on ER membrane and to maintain its integrity. ERIS was dimerized on innate immune challenges. Coumermycin-induced ERIS dimerization led to strong and fast IFN induction, suggesting that dimerization of ERIS was critical for self-activation and subsequent downstream signaling.

• 出版日期2009-5-26
• 单位北京大学