Ampelopsin attenuates the atrophy of skeletal muscle from D-gal-induced aging rats through activating AMPK/SIRT1/PGC-1 alpha signaling cascade

作者：Kou, Xianjuan; Li, Jie; Liu, Xingran; Yang, Xiaoqi; Fan, Jingjing; Chen, Ning^*

来源：Biomedicine & Pharmacotherapy, 2017, 90: 311-320.

DOI：10.1016/j.biopha.2017.03.070

• Ampelopsin
• Skeletal muscle atrophy
• D-Galactose
• Autophagy
• p-AMPK-PGC-1 alpha signal pathway

The atrophy of skeletal muscle is highly correlated with oxidative damage, excessive apoptosis and dysfunctional autophagy. Ampelopsin, a natural flavonoid, has multiple biological functions including anti-inflammatory, anti-oxidative, and hepatoprotective functions. Sprague-Dawley (SD) rats subjected to intraperitoneal injection of D-galactose (D-gal) at the dose of 150 mg/kg.d revealed an obvious atrophy of skeletal muscle with significantly reduced muscle mass/body mass ratio, cross-sectional area and fiber diameter of skeletal muscle in D-gal-induced aging rats when compared to normal control rats without D-gal administration for 6 consecutive weeks. In contrast, the combinatorial administration of D-gal at the identical dose and DHM at the dose of 100 or 200 mg/kg.d could alleviate the reduction of these hallmarks associated with the atrophy of skeletal muscle. In addition, D-gal administration could result in obvious apoptosis and impaired autophagy in skeletal muscle, which could be mitigated upon DHM treatment due to its role in decreasing ubiquitin and Atrogin-1/MAFbx and up-regulating AMPK and SIRT1 signal pathways. Therefore, DHM may be a potential candidate for the prevention and treatment of skeletal muscle atrophy associated aging process.

• 出版日期2017-6
• 单位武汉体育学院