Development of a recombinant camelid specific diabody against the heminecrolysin fraction of Hemiscorpius lepturus scorpion

作者：Bagheri Maryam; Babaei Esmaeil; Shahbazzadeh Delavar; Habibi Anbouhi Mahdi; Alirahimi Ehsan; Kazemi Lomedasht Fatemeh; Behdani Mahdi^*

来源：Toxin Reviews, 2017, 36(1): 7-11.

DOI：10.1080/15569543.2016.1244552

• Hemiscorpius lepturus
• scorpion
• diabody
• nanobody
• envenomation

Scorpion sting is one of the most important problems in public health practice in many countries. Khuzestan yellow scorpion, scientifically known as Hemiscorpius lepturus, belongs to the family Hemiscorpidae, and is one of the most common and dangerous scorpions in Iran. Hemiscorpius lepturus scorpion has potentially cytotoxic venom. The first treatment for scorpion envenoming is horse antisera. Due to their unique characteristics, camels have been considered as an important source for antiserum and monoclonal antibody (Nanobody) production. In the previous study, we reported a specific nanobody against the hemolytic fraction (heminecrolysin) of Hemiscorpius lepturus venom. In the present study, a recombinant diabody was constructed and produced by fusion of two nanobodies. Results from in vitro and in vivo assays showed that the diabody has a promising ability to neutralize venom activity.

• 出版日期2017