A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production

作者:Tossi Vanesa E; Acebedo Sofia L; Cassia Raul O; Lamattina Lorenzo; Galagovsky Lydia R; Ramirez Javier A*
来源:Steroids, 2015, 102: 46-52.
DOI:10.1016/j.steroids.2015.07.003

摘要

Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies.

  • 出版日期2015-10