Amino Acid Substitution at Position 95 in Rabies Virus Matrix Protein Affects Viral Pathogenicity

作者：Ito Naoto; Mita Tetsuo; Shimizu Kenta; Ito Yuki; Masatani Tatsunori; Nakagawa Keisuke; Yamaoka Satoko; Abe Masako; Okadera Kota; Minamoto Nobuyuki; Sugiyama Makoto^*

来源：Journal of Veterinary Medical Science, 2011, 73(10): 1363-1366.

DOI：10.1292/jvms.11-0151

• matrix protein
• pathogenicity
• rabies virus

We previously reported that rabies virus strain CE(NiM), but not the parental Ni-CE strain, killed mice after intracerebral inoculation. CE(NiM) and Ni-CE are genetically identical except for two amino acids at positions 29 and 95 in the M protein. In this study, to identify which residue determines the pathogenicity, we examined pathogenicities of two Ni-CE mutants, CE(NiM29) and CE(NiM95), which were established by replacement of an amino acid residue at position 29 or 95 in the Ni-CE M protein with the corresponding residue CE(NiM), respectively. We found that CE(NiM95), but not CE(NiM29), killed mice, indicating that the amino acid at position 95 in the M protein is the pathogenic determinant.

• 出版日期2011-10