Mushroom tyrosinase inhibitors from mung bean (Vigna radiatae L.) extracts

作者：Yao, Yang; Cheng, Xuzhen; Wang, Lixia; Wang, Suhua; Ren, Guixing^*

来源：International Journal of Food Sciences and Nutrition, 2012, 63(3): 358-361.

DOI：10.3109/09637486.2011.629177

• vitexin
• isovitexin
• IC50

A seventy percent ethanol from mung bean (Vigna radiatae L.) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. When using L-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase, the EtOAc-soluble fractions showed the highest inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guided fractionation method) from the EtOAc-soluble fractions. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 6.3 and 5.6 mg/ml, respectively. This is the first study on the active compositions of azuki beans against mushroom tyrosinase.

• 出版日期2012-5
• 单位中国农业科学院