科研之友
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摘要
Objective-In severe stenosis, von Willebrand Factor (vWF) experiences millisecond exposures to pathological wall shear rates (gamma(w)). We sought to evaluate the deposition of vWF onto collagen surfaces under flow in these environments. Methods and Results-Distinct from viscometry experiments that last many seconds, we deployed microfluidic devices for single-pass perfusion of whole blood or platelet-free plasma over fibrillar type 1 collagen (<50 ms transit time) at pathological gamma(w) or spatial wall shear rate gradients (grad gamma(w)). Using fluorescent anti-vWF, long thick vWF fibers (>20 mu m) bound to collagen were visualized at constant gamma(w)>30 000 s(-1) during perfusion of platelet-free plasma, a process enhanced by EDTA. Rapid acceleration or deceleration of EDTA platelet-free plasma at grad gamma(w)=+/- 1.1x10(5) to +/- 4.3x10(7) s(-1)/cm did not promote vWF deposition. At 19 400 s(-1), EDTA blood perfusion resulted in rolling vWF-platelet nets, although blood perfusion (normal Ca2+) generated large vWF/platelet deposits that repeatedly embolized and were blocked by anti-glycoprotein Ib or the alpha(IIb)beta(3) inhibitor GR144053 and did not require grad gamma(w). Blood perfusion at venous shear rate (200 s(-1)) produced a stable platelet deposit that was a substrate for massive but unstable vWF-platelet aggregates when flow was increased to 7800 s(-1). Conclusion-Triggered by collagen and enhanced by platelet glycoprotein Ib and alpha(IIb)beta(3), vWF fiber formation occurred during acute exposures to pathological gamma(w) and did not require gradients in wall shear rate. (Arterioscler Thromb Vasc Biol. 2013;33:105-113.)
- 出版日期2013-1
- 单位河北医科大学
