Uridine phosphorylase from Shewanella oneidensis MR-1: Heterological expression, regulation, transcription, and properties

作者：Mordkovich N N^*; Manuvera V A; Veiko V P; Debabov V G

来源：Applied Biochemistry and Microbiology, 2012, 48(9): 716-722.

DOI：10.1134/S0003683812090037

• heterological expression
• uridine phosphorylase (UDP)
• Escherichia coli
• Shewanella oneidensis

A DNA fragment containing a promoter-operator and structural parts of the uridine phosphorylase gene from Shewanella oneidensis MR-1 was cloned. Cross-heterological expression of the udp genes from Sh. oneidensis MR-1 and Escherichia coli under the control of authentic regulatory regions is shown. The UDP protein accumulates in an active form in the cytoplasmic fraction of cells. The recombinant UDP protein from Sh. oneidensis MR-1 obtained by heterological expression was isolated and characterized. E. coli udp gene promoter activity was observed during heterological expression in Sh. oneidensis MR-1 cells under both aerobic and anaerobic conditions.

• 出版日期2012-12