Direct electrogenerated chemiluminescence detection in high-performance liquid chromatography for determination of ofloxacin

作者：Sun Yonghua; Zhang Zhujun^*; Xi Zhijun

来源：Analytica Chimica Acta, 2008, 623(1): 96-100.

DOI：10.1016/j.aca.2008.06.006

• direct electrogenerated chemiluminescence
• liquid chromatography
• dual-electrode system
• ofloxacin

Ofloxacin (OFLX) exhibited strong electrogenerated chemiluminescence (ECL) in NaNO3 solution with a dual-electrode system when constant current was exerted. Based on this observation, a sensitive direct ECL method coupled with high-performance liquid chromatography (HPLC) separation was developed for determination of OFLX in human serum. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of OFLX in the range of 1.0 x 10(-8) to 4.0 x 10(-6) g mL(-1) and the detection limit was 4 x 10(-9) g mL(-1) (S/N = 3). The proposed method was sensitive, simple and convenient to operate.

• 出版日期2008-8-8
• 单位陕西师范大学