Downregulation of N-methyl-D-aspartate receptor zeta 1 subunit (GluN1) gene in inferior colliculus with aging

作者：Osumi Yasunori; Shibata Seiji Bruce; Kanda Seiji^*; Yagi Masao; Ooka Hisashi; Shimano Takashi; Asako Mikiya; Kawamoto Kohei; Kuriyama Hiromichi; Inoue Toshiya; Nishiyama Toshimasa; Yamashita Toshio; Tomoda Koichi

来源：Brain Research, 2012, 1454: 23-32.

DOI：10.1016/j.brainres.2012.03.018

• Presbycusis
• Hearing loss
• Aging
• Inferior colliculus
• Gene expression

Presbycusis is the impairment of auditory function associated with aging, which stems from peripheral cochlear lesions and degeneration of the central auditory process. The effect of age-induced peripheral hearing loss on the central auditory process is not fully understood. C57B1/6 (C57) mice present accelerated peripheral hearing loss, which is well developed by middle-age and mimics the human presbycusis pattern. The aim of this study was to elucidate the molecular effects of peripheral hearing loss in the inferior colliculus (IC) with age between young and middle-aged C57 mice using cDNA microarray. Glutamate receptor ionotropic NMDA zeta 1 (GluN1) exhibited the greatest decrease in the middle-aged group as determined using cDNA microarray and by further assessment using real-time PCR (qPCR). Histological assessment with in situ hybridization of GluN1 showed significantly decreased expression in all IC subdivisions of the middle-aged group. GluN1 is a receptor for excitatory neurotransmission, and significant downregulation of this gene may be subsequent to the decline of afferent input from the cochlea in aging C57 mice. Consequently, using the combination of microarray, qPCR, and in situ hybridization, we showed that the decline of GluN1 in the IC of aging animals might have a key role in the pathogenesis of presbycusis.

• 出版日期2012-5-15