Use of high-resolution melting curve analysis to differentiate vaccine and wild type strains of grass carp reovirus genotype II

作者：Guo, Yanmin; Zeng, Weiwei^*; Wang, Qing; Wang, Yingying; Li, Yingying; Yin, Jiyuan; Ren, Yan; Shi, Cunbin

来源：Journal of Virological Methods, 2018, 256: 111-115.

DOI：10.1016/j.jviromet.2018.03.006

• Grass carp reovirus
• High resolution melting (HRM)
• qPCR
• Differential diagnosis

Grass carp reovirus (GCRV) is the causative agent of a hemorrhagic disease that causes severe economic losses in the grass carp (Ctenopharyngodon idella) farming industry in China. Discrimination between wild-type field and vaccine strains of GCRV is crucial for meaningful disease diagnosis and epidemiological investigation, yet current detection methods do not discriminate between these different viruses. This study exploited sequence differences between vaccine viruses and the virulent and field strains in the S6 gene that is present in all GCRV strains to develop a high resolution melting curve assay to differentiate between virus strains. The high resolution melting curve analysis was as analytically sensitive as real-time quantitative-Polymerase Chain Reaction (qPCR) detection and at least 10 times sensitive than the conventional PCR. This one-step assay will facilitate grass carp hemorrhagic disease outbreak responses and control.

• 出版日期2018-6
• 单位菏泽学院; 中国水产科学研究院珠江水产研究所