Analysis of Glycosaminoglycan Oligosaccharides by Combined HPTLC/MALDI-TOF MS: Reduced Silica Gel Thickness Leads to Improved Spectral Qualities and Reduced Side Reactions

作者：Lemmnitzer Katharina; Griesinger Hans; Suess Rosmarie; Matheis Katerina; Schulz Michael; Schiller Juergen^*

来源：Chromatographia, 2015, 78(21-22): 1409-1413.

DOI：10.1007/s10337-015-2971-2

• Oligosaccharides
• Hyaluronan
• Chondroitin sulfate
• MALDI-TOF MS
• HPTLC
• Formylation

Thin-layer chromatography (TLC) is a simple, fast and inexpensive separation method which can be applied to virtually all natural products including oligosaccharides. Unfortunately, however, the unequivocal identification of a TLC spot is normally difficult. Fortunately, this problem can be minimized when mass spectrometry (MS) such as matrix-assisted laser desorption and ionization time-of-flight is used to identify the TLC spots. This work is dedicated to the TLC/MS analysis of oligosaccharides derived from native chondroitin sulfate and hyaluronan. We will show that the thickness of the silica gel layer (200 versus 100 A mu m) has a tremendous influence on the quality of the mass spectra: a reduced silica gel thickness enhances the spectral quality and, in particular, improves the achievable signal-to-noise ratio. Additionally, unwanted formylation of the GAG oligosaccharides (which occurs due to the high moiety of formic acid in the mobile phase) can also be minimized if MS-grade HPTLC plates are used.

• 出版日期2015-11
• 单位河北医科大学