High-fat diet-induced reduction of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha messenger RNA levels and oxidative capacity in the soleus muscle of rats with metabolic syndrome

作者：Nagatomo Fumiko; Fujino Hidemi; Kondo Hiroyo; Takeda Isao; Tsuda Kinsuke; Ishihara Akihiko^*

来源：Nutrition Research, 2012, 32(2): 144-151.

DOI：10.1016/j.nutres.2011.12.015

• Muscle fiber type
• Muscle oxidative enzyme activity
• Obesity
• Skeletal muscle

Animal models of type 2 diabetes exhibit reduced peroxisome proliferator activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) messenger RNA (mRNA) levels, which are associated with decreased oxidative capacity, in skeletal muscles. In contrast, animal models with metabolic syndrome show normal PGC-1 alpha mRNA levels. We hypothesized that a high-fat diet decreases PGC-1 alpha mRNA levels in skeletal muscles of rats with metabolic syndrome, reducing muscle oxidative capacity and accelerating metabolic syndrome or inducing type 2 diabetes. We examined mRNA levels and fiber profiles in the solcus muscles of rats with metabolic syndrome (SHR/NDmer-cp [cp/cp]; CP) fed a high-fat diet. Five-week-old CP rats were assigned to a sedentary group (CP-N) that was fed a standard diet (15.1 kJ/g, 23.6% protein, 5.3% fat, and 54.4% carbohydrates) or a sedentary group (CP-H) that was fed a high-fat diet (21.6 kJ/g, 23.6% protein, 34.9% fat, and 25.9% carbohydrates) and were housed for 10 weeks. Body weight, energy intake, and systolic blood pressure were higher in the CP-H group than in the CP-N group. Non fasting glucose, triglyceride, total cholesterol, and leptin levels were higher in the CP-H group than in the CP-N group. There was no difference in insulin levels between the CP-N and CP-H groups. Muscle PGC-1 alpha mRNA levels and succinate dehydrogenase activity were lower in the CP-H group than in the CP-N group. We concluded that a high-fat diet reduces PGC-1 alpha mRNA levels and oxidative capacity in skeletal muscles and accelerates metabolic syndrome.

• 出版日期2012-2