Fast determination of arsenosugars in algal extracts by narrow bore high-performance liquid chromatography-inductively coupled plasma mass spectrometry

作者：Luis Todoli Jose; Grotti Marco^*

来源：Journal of Chromatography A, 2010, 1217(47): 7428-7433.

DOI：10.1016/j.chroma.2010.09.065

• Narrow bore HPLC
• ICP-MS
• Micro-liquid chromatography
• Anion exchange
• Speciation
• Arsenic
• Arsenosugars

The potential of narrow bore high-performance liquid chromatography (HPLC) with detection by inductively coupled plasma mass spectrometry (ICP-MS) for fast determination of arsenosugars in algal extracts was explored. The retention behavior of four naturally occurring dimethylarsinoylribosides on an anion-exchange microbore column was investigated, with the mobile phase flow rate ranging from 60 to 200 mu L min(-1). A low sample consumption system consisting of a micronebulizer and a low inner volume cyclonic spray chamber was used as the interface between the micro-column and the ICP mass spectrometer. Both the high efficiency nebulizer, HEN, and the PFA micronebulizer were tested, with the former providing 20-50% greater sensitivity than PFA (depending on the liquid flow rate), but comparable limits of detection and slightly lower chromatographic resolution. With the setup employed and under the optimal conditions, a satisfactory separation of the arsenosugars was achieved in less than 5 min. The instrumental limit of detection was 0.20 mu g As L(-1) and the precision was better than 3% (RSD%, n = 5). The accuracy of the determination was verified by the analysis of a reference algal extract, obtaining values in good agreement with the reference ones.

• 出版日期2010-11-19