Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice

作者：Chao Jung; Lu Tsung Chun; Liao Jiunn Wang; Huang Tai Hung; Lee Meng Shiou; Cheng Hao Yuan; Ho Li Kang; Kuo Chao Lin; Peng Wen Huang^*

来源：Journal of Ethnopharmacology, 2009, 125(2): 297-303.

DOI：10.1016/j.jep.2009.06.024

• Mahonia oiwakensis
• Berberidaceae
• Anti-inflammation
• Analgesia
• Carrageenan
• Acetic acid-induced writhing response
• Formalin test

Aims of the study: This study investigated the anti-inflammatory and analgesic activities, and protoberberine alkaloid contents of ethanol extract of MO roots (MOR(EtOH)).
Materials and methods: The analgesic activity of MOR(EtOH) was determined using acetic acid-induced writhing response and formalin test. The anti-inflammatory activity of MOR(EtOH) was determined using the lambda-carrageenan-induced paw oedema model. The protoberberine alkaloid contents of MOR(EtOH) were identified by high-performance liquid chromatography (HPLC).
Results: MOR(EtOH) (100 and 500 mg/kg) decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, carrageenan-induced paw oedema was significantly reduced in a dose-dependent manner by administering MOR(EtOH) (100 and 500 mg/kg) at 3, 4, and 5 h after the carrageenan injection. The serum levels of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) of MOR(EtOH)-treated mice were significantly reduced compared with those in the serum of animals administered carrageenan. Notably, MOR(EtOH) attenuated the expression of cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) and neutrophil infiltration in paw tissues injected with carrageenan. The anti-inflammatory mechanisms of MOR(EtOH) appear to be related to the inhibition of neutrophil infiltration, iNOS and COX-2 protein expression, NO release, and the decreasing TNF-alpha level in serum. The analytical results showed that the contents of berberine, palmatine and jatrorrhizine were 191.45 mg/g extract, 100.15 mg/g extract and 66.45 mg/g extract, respectively.
Conclusion: These experimental results suggest that MOR(EtOH) produced both analgesic and anti-inflammatory effects in mice and may be a candidate for the development of pharmacological agents used in the treatment of inflammatory disorders. Published by Elsevier Ireland Ltd

• 出版日期2009-9-7
• 单位中国医科大学