How half sandwich ruthenium compounds interact with DNA while not being hydrolyzed; a comparative study

作者：Tsolis Theodoros; Papavasileiou Konstantinos D; Divanis Spyridon A; Melissas Vasilios S; Garoufis Achilleas^*

来源：Journal of Inorganic Biochemistry, 2016, 160: 12-23.

DOI：10.1016/j.jinorgbio.2016.04.008

• Ruthenium anticancer compounds
• Non-coordinative interactions
• Oligonucleotide
• d(5 '-CGCGCG-3 ')(2)
• Molecular docking

The non-coordinative interactions between the compounds (eta(6)-cym)Ru(pqx)Cl]Cl, (1)Cl, [(eta(6)-cym)Ru(pbqx)Cl]Cl, (2)Cl (cym is 1-methyl-4-(1-methylethyl)benzene, pqx is 2-(2'-pyridyl)quinoxaline and pbqx is 2-(2'-pyridyl)benzo[g]quinoxaline), with the self-complementary oligonucleotide duplex d(5'-CGCG CG-3')(2) in 100 mM phosphates buffer and 50 mM NaCl, were studied by means of NMR spectroscopic techniques. The compounds (1)0 and (2)Cl were found to be stable in aqueous media without being hydrolyzed, preserving their saturated coordination sphere (1)eta(6)-arene-2N-Cl). Based on NMR techniques it was found that both (1) and (2) interact with the hexamer with the outer side of the chelating ligand L, causing elongation or disruption of the oligonucleotide Watson-Crick (W-C) hydrogen bonds. Since both compounds are highly cytotoxic, it can be concluded that their cytotoxic activity could be owed to this type of interactions, or non-coordinative interactions with other biomolecules.

• 出版日期2016-7