Determination of Multimodal Isotopic Distributions: The Case of a N-15 Labeled Protein Produced into Hairy Roots

作者：Trouillard Romain; Hubert Roux Marie; Tognetti Vincent; Guilhaudis Laure; Plasson Carole; Menu Bouaouiche Laurence; Coquet Laurent; Guerineau Francois; Hardouin Julie; Ekouna Jean Pierre Ele; Cosette Pascal; Lerouge Patrice; Boitel Conti Michele; Afonso Carlos^*; Segalas Milazzo Isabelle

来源：Analytical Chemistry, 2015, 87(12): 5938-5946.

DOI：10.1021/acs.analchem.5b01558

Isotopic labeling is widely used in various fields like proteomics, metabolomics, fluxomics, as well as in NMR structural studies, but it requires an efficient determination of the isotopic enrichment. Mass spectrometry is the method of choice for such analysis. However, when complex expression systems like hairy roots are used for production, multiple populations of labeled proteins maybe obtained. If the isotopic incorporation determination is actually well-known for unimodal distributions, the multimodal distributions have scarcely been investigated. Actually, only,a few approaches allow the determination of the different labeled population proportions from multimodal distributions. Furthermore, they cannot be used when the number of the populations and their respective isotope ratios are unknown. The present study implements a new strategy to measure the N-15 labeled populations inside a multimodal distribution knowing only the peptide sequence and peak intensities from mass spectrometry analyses. Noteworthy, it could be applied to other elements, like carbon and hydrogen, and extended to a larger range of biomolecules.

• 出版日期2015-6-16