A novel method for the detection of Roundup Ready (TM) soya using liquid chromatography-electrospray ionisation mass spectrometry

作者：Shanahan Della^*; Stokes Peter; Burns Malcolm; Harris Neil

来源：European Food Research and Technology, 2007, 225(3-4): 483-491.

DOI：10.1007/s00217-006-0444-4

• genetically modified
• mass spectrometry
• Roundup Ready (TM) soya
• LC-MS
• primer extension

Current methods for the detection and quantification of genetically modified (GM) ingredients predominantly make use of enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) based techniques. For PCR-based techniques, real-time detection systems represent the state of the art for quantitative measurement of DNA-based products. This study sought to assess the viability of using mass spectrometry (MS) for the detection and potential quantification of GM-derived material by developing an assay for the detection of Roundup Ready (TM) soya (RRS). This was based upon the indirect detection of event-specific PCR products using a single base pair extension followed by online clean-up and detection using liquid chromatography-electrospray-mass spectrometry (LC- ESI-MS). We were able to demonstrate that LC-ESI-MS detection provides conclusive identification of the primer extension products enabling the effective detection and differentiation of GM-and non-GM-derived material. This was carried out using both reference materials and real food matrices.

• 出版日期2007-7