Nicotine, acetanilide and urea multi-level H-2-, C-13- and N-15-abundance reference materials for continuous-flow isotope ratio mass spectrometry

作者：Schimmelmann Arndt^*; Albertino Andrea; Sauer Peter E; Qi Haiping; Molinie Roland; Mesnard Francois

来源：Rapid Communications in Mass Spectrometry, 2009, 23(22): 3513-3521.

DOI：10.1002/rcm.4277

Accurate determinations of stable isotope ratios require a calibration using at least two reference materials with different isotopic compositions to anchor the isotopic scale and compensate for differences in machine slope. Ideally, the delta values of these reference materials should bracket the isotopic range of samples with unknown delta values. While the practice of analyzing two isotopically distinct reference materials is common for water (VSMOW-SLAP) and carbonates (NBS 19 and L-SVEC), the lack of widely available organic reference materials with distinct isotopic composition has hindered the practice when analyzing organic materials by elemental analysis/isotope ratio mass spectrometry (EA-IRMS). At present only L-glutamic acids USGS40 and USGS41 satisfy these requirements for delta C-13 and delta N-15, with the limitation that L-glutamic acid is not suitable for analysis by gas chromatography (GC). We describe the development and quality testing of (i) four nicotine laboratory reference materials for on-line (i.e. continuous flow) hydrogen reductive gas chromatography-isotope ratio mass-spectrometry (GC-IRMS), (ii) five nicotines for oxidative C, N gas chromatography-combustion-isotope ratio mass-spectrometry (GC-C-IRMS, or GC-IRMS), and (iii) also three acetanilide and three urea reference materials for on-line oxidative EA-IRMS for C and N. Isotopic off-line calibration against international stable isotope measurement standards at Indiana University adhered to the 'principle of identical treatment'. The new reference materials cover the following isotopic ranges: delta H-2(nicotine) -162 to -45 parts per thousand, delta C-13(nicotine) -30.05 to +7.72 parts per thousand, delta N-15(nicotine) -6.03 to +33.62 parts per thousand; delta N-15(acetanilide) +1.18 to +40.57 parts per thousand; delta C-13(urea) -34.13 to +11.71 parts per thousand, delta N-15(urea) +0.26 to +40.61 parts per thousand (recommended delta values refer to calibration with NBS 19, L-SVEC, IAEA-N-1, and IAEA-N-2). Nicotines fill a gap as the first organic nitrogen stable isotope reference materials for GC-IRMS that are available with different delta N-15 values. Comparative delta C-13 and delta N-15 on-line EA-IRMS data from 14 volunteering laboratories document the usefulness and reliability of acetanilides and ureas as EA-IRMS reference materials. Published in 2009 by John Wiley & Sons, Ltd.

• 出版日期2009-11