Antiproliferation and apoptosis induced by tamoxifen in human bile duct carcinoma QBC939 cells via upregulated p53 expression

作者：Han Peng; Kang Jin He; Li Hua Liang; Hu Su Xian; Lian Hui Hui; Qiu Ping Ping; Zhang Jian; Li Wen Gang^*; Chen Qing Xi

来源：Biochemical and Biophysical Research Communications, 2009, 385(2): 251-256.

DOI：10.1016/j.bbrc.2009.05.059

• Tamoxifen
• Bile duct carcinoma
• Antiproliferation
• Apoptosis
• p53

Tamoxifen (TAM) is a nonsteroidal antiestrogen that has been used in the treatment of breast cancer for over 30 years. Recently, it was shown that TAM also has efficacy on gastrointestinal neoplasms such as hepatocarcinoma and pancreatic carcinoma, and that the chemopreventive activities of TAM might be due to its abilities to inhibit cell growth and induce apoptosis. In the present study, we investigated the effects of tamoxifen on growth and apoptosis in the human bile duct carcinoma (BDC cell line QBC939 using MTT assay, inverted microscopy, fluorescence microscopy, transmission election microscopy. classic DNA fragmentation agarose gel electrophoresis assay, PI single- and FITC/PI double-staining flow cytometry, and Western blotting. Our data revealed that TAM could significantly inhibit growth and induce apoptosis in QBC939 cells. Increased expression of p53 was observed in TAM-treated cells, indicating that p53 might play an important role in TAM-induced apoptosis in QBC939 cells. These results provide significant insight into the anticarcinogenic action of TAM on BDC.

• 出版日期2009-7-24
• 单位厦门大学; 福建医科大学