A histological evaluation on osteogenesis and resorption of methotrexate-loaded calcium phosphate cement in vivo

作者：Li, Dong; Yang, Zhiping^*; Li, Xin; Li, Zhenfeng; Li, Jianmin; Yang, Jingyan

来源：Biomedical Materials (Bristol), 2010, 5(2): 025007.

DOI：10.1088/1748-6041/5/2/025007

• BETA-TRICALCIUM PHOSPHATE
• DRUG-DELIVERY SYSTEM
• BONE-FORMATION
• RELEASE
• BIOMATERIALS
• IMPLANTATION
• VITRO

In this study, we investigated the resorption of in vivo methotrexate-loaded calcium phosphate cement (MTX-CPC) implants and their effect on osteogenesis. MTX-CPC implants containing 1% methotrexate (MTX) (weight/weight) were preset and implanted into the femoral condyle of rabbits. Calcium phosphate cement (CPC) without MTX was used as the control. The femurs were harvested at day 1 and at 1, 3 and 6 months after implantation and radiological examination were performed. Decalcified sections were examined by hematoxylin and eosin ( HE) staining, alkaline phosphatase (ALPase) immunohistochemistry and tartrate-resistant acid phosphatase (TRAPase) enzyme histochemistry. Then, we performed histomorphometric analysis, including determination of the percentage of newly formed bone and osteoblast and osteoclast counts. The results indicated that MTX-CPC implants were biocompatible, biodegradable and osteoconducive. However, MTX release from the implantation site inhibited osteogenesis in the initial period; this inhibition weakened with time, and no difference was observed between CPC and MTX-CPC at 6 months after implantation. Hence, MTX-CPC is an excellent material for filling defects and can be used for preparing effective drug delivery systems to achieve local control of invasive bone tumors.

• 出版日期2010-4
• 单位山东大学