Proton motive force generation from stored polymers for the uptake of acetate under anaerobic conditions

作者：Saunders Aaron M; Mabbett Amanda N; McEwan Alastair G; Blackall Linda L^*

来源：FEMS Microbiology Letters, 2007, 274(2): 245-251.

DOI：10.1111/j.1574-6968.2007.00839.x

• enhanced biological phosphorus removal
• polyphosphate
• polyhydroxyalkanoate
• secondary transport
• substrate uptake

The bacteria facilitating enhanced biological phosphorus removal gain a selective advantage from intracellularly stored polymer-driven substrate uptake under anaerobic conditions during sequential anaerobic : aerobic cycling. Mechanisms for these unusual membrane transport processes were proposed and experimentally validated using selective inhibitors and highly-enriched cultures of a polyphosphate-accumulating organism, Accumulibacter, and a glycogen-accumulating organism, Competibacter. Acetate uptake by both Accumulibacter and Competibacter was driven by a proton motive force (PMF). Stored polymers were used to generate the PMF - Accumulibacter used phosphate efflux through the Pit transporter, while Competibacter generated a PMF by proton efflux through the ATPase and fumarate reductase in the reductive TCA cycle.

• 出版日期2007-9