USE OF LINEAR EQUATIONS TO OBTAIN MICHAELIS-MENTEN PARAMETERS

作者：Carvalho Nakedia M F^*; Pires Bianca M; Antunes Octavio A C; Faria Roberto B; Osorio Renata E H M B; Piovezan Clovis; Neves Ademir

来源：Quimica Nova, 2010, 33(7): 1607-1611.

DOI：10.1590/S0100-40422010000700033

• Michaelis-Menten
• Lineweaver-Burk
• nonlinear least-square fit

USE OF LINEAR EQUATIONS TO OBTAIN MICHAELIS-MENTEN PARAMETERS. The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K(M), V(max), and k(cat) by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.

• 出版日期2010