A highly sensitive quartz crystal microbalance immunosensor based on magnetic bead-supported bienzymes catalyzed mass enhancement strategy

作者：Akter Rashida; Rhee Choong Kyun^*; Rahman Md Aminur

来源：Biosensors and Bioelectronics, 2015, 66: 539-546.

DOI：10.1016/j.bios.2014.12.007

• Quartz crystal microbalance
• Immunosensor
• Magnetic bead-supported bioconjugate
• Biocatalytic precipitation
• Protein detection
• Human Immunoglobulin

A highly sensitive quartz crystal microbalance (QCM) immunosensor based on magnetic bead-supported bienzyme catalyzed mass enhanced strategy was developed for the detection of human immunoglobulin G (hIgG) protein. The high sensitive detection was achieved by increasing the deposited mass on the QCM crystal through the enhanced precipitation of 4-chloro-1-naphthol (CN) using higher amounts of horseradish peroxidase (HRP) and glucose oxidase (GOx) bienzymes attached on the magnetic beads (MB). The protein A (PA) and capture antibody (monoclonal anti-human IgG antibody produced in mouse, Ab(1))-based QCM probe and the detection antibody (anti-human IgG antibody produced in goat, Ab(2))-based MB/HRP/GO(x) bienzymatic bioconjugates were characterized using scanning electron microscope, transmission electron microscope, cyclic voltammetry, and electrochemical impedance spectroscopy techniques. Under the optimized experimental condition, the linear range and the detection limit of hIgG immunosensor were determined to be 5.0 pg/mL-20.0 ng/mL and 5.0 +/- 0.18 pg/mL, respectively. The applicability of the present hIgG immunosensor was examined in hIgG spiked human serum samples and excellent recoveries of hIgG were obtained.

• 出版日期2015-4-15