A novel PBT-based fluorescent probe for hydrazine detection and its application in living cells

作者：Xu, Wenzhi; Liu, Weiyan; Zhou, Tingting; Yang, Yutao^*; Li, Wei^*

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2018, 356: 610-616.

DOI：10.1016/j.jphotochem.2018.02.004

• Probe
• Hydrazine
• 2-Phenyl-benzothiazole
• Fluorescence
• Sensitivity
• Bioimaging

A new 2-phenyl-benzothiazole (PBT) based fluorescent probe (AcO-PBT-CHO) was designed and synthesized for the detection of hydrazine. The aldehyde and acetyl ester groups were used as the recognition units. Upon the treatment of the probe with hydrazine, the emission at 505 nm decreased while the emission at 430 nm increased. Accordingly, the fluorescent color changed from green to blue. The aldehyde and acetyl ester groups exerted additive effect on the fluorescence spectrum changes of the probe. The probe could selectively identify hydrazine over other related interfering species. The probe could detect hydrazine quantitatively in the range of 20-40 mu M with the detection limit of 2.80 ppb. Furthermore, the probe was successfully applied to detect hydrazine in living cells.

• 出版日期2018-4-1
• 单位河北大学