Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms

作者：Morishita Soji; Komatsu Norio; Kirito Keita; Koda Aya H; Sekiguchi Yuji; Tsuneda Satoshi; Noda Naohiro^*

来源：Leukemia Research, 2011, 35(12): 1632-1636.

DOI：10.1016/j.leukres.2011.06.016

• Myeloproliferative neoplasms
• JAK2V617F mutation
• Allele burden
• Fluorescence quenching
• Gene quantification

We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

• 出版日期2011-12