A series of novel NIR fluorescent dyes: Synthesis, theoretical calculations and fluorescence imaging applications in living cells

作者：Zhang, Ming; Zheng, Shu-ting; Liu, Xiao-jing; Long, Ying; Yang, Bing-qin^*

来源：Dyes and Pigments, 2016, 125: 220-228.

DOI：10.1016/j.dyepig.2015.10.028

• NIR fluorescent dyes
• Stokes shifts
• Biological imaging
• Cytotoxicity
• Theoretical calculations
• Fluorescent sensor

A series of NIR fluorescent dyes have been synthesized and characterized. These new dyes displayed excellent photo-physical properties including large Stokes shifts, high fluorescence quantum yields, good photo-stability and low cytotoxicity. Significantly, these novel NIR dyes are superior to the traditional rhodamine dyes, displaying strong emission in the NIR region as well as the rhodamine-like ON-OFF fluorescence switching mechanism. In addition, using one of the new dyes as a platform, a novel Hg2+ fluorescent sensor was constructed which was capable of imaging Hg2+ in living cells, demonstrating the practical value of these new dyes. Finally, to understand the mechanism of action of the sensors and their enhanced fluorescence properties, we performed quantum chemical calculations with the B3LYP exchange functional employing 6-31G basis.

• 出版日期2016-2
• 单位西北大学