A simple electrokinetic protein preconcentrator utilizing nano-interstices

作者：Chen Yu Hung^*; Wu Hsuan Franziska; Amstislavskaya Tamara G; Li Chang Yu; Jen Chun Ping^*

来源：Biomicrofluidics, 2016, 10(2): 024121.

DOI：10.1063/1.4946768

This work proposes a simple method for creating nanofluidic channels for protein preconcentration through self-assembled gold nanoparticles (AuNPs) using the exclusion-enrichment effect. A depletion force is elicited in nano-interstices among self-assembled AuNPs due to the overlap of electrical double layers (EDLs); therefore, proteins quickly accumulate. The experimental results show that the generation of depletion forces is correlated with the size of the AuNPs. The self-assembled monolayer of AuNPs (13 nm in diameter) can successfully preconcentrate proteins through effective EDL overlapping. This approach provides a new process to produce nanochannels that does not require high-voltage or time-consuming fabrication. Published by AIP Publishing.

• 出版日期2016-3