A new arginine-based dental adhesive system: formulation, mechanical and anti-caries properties

作者：Geraldeli Saulo; Soares Eveline F; Alvarez Andres J; Farivar Tanaz; Shields Robert C; Sinhoreti Mario A C; Nascimento Marcelle M^*

来源：Journal of Dentistry, 2017, 63: 72-80.

DOI：10.1016/j.jdent.2017.05.024

• Arginine
• Dental adhesives
• Oral biofilms
• Caries
• Secondary caries

Secondary caries at the margins of composite restorations has been attributed to adhesive failure and consequent accumulation of cariogenic biofilms.
Objectives: To develop and evaluate an etch-and-rinse adhesive system containing arginine for sustainable release and recharge without affecting its mechanical properties. Arginine metabolism by oral bacteria generates ammonia, which neutralizes glycolytic acids and creates a neutral environmental pH that is less favorable to the growth of caries pathogens, thus reducing the caries risk at the tooth-composite interface.
Methods: Experimental adhesives were formulated with methacrylate monomers and arginine at 5%, 7%, and 10% or no arginine (control). Adhesives were tested for: (i) mechanical properties of true stress (FS and UTS), modulus of elasticity (E), degree of conversion (DC), Knoop hardness number (KHN) and dentin microtensile bond strength (mu-TBS), (ii) arginine release and recharge, and (iii) antibacterial activities. Data was analyzed by t-test, one-way ANOVA and Tukey's tests.
Results: FS and UTS results showed no statistically significant differences between the 7% arginine-adhesive and control, while the results for E, DC, KHN and mu-TBS showed no difference among all groups. The 7% arginineadhesive showed a high release rate of arginine (75.0 mu mol/cm(2)) at 2 h, and a more sustainable, controlled release rate (up to 0.2 mu mol/cm(2)) at 30 days.
Conclusions: Incorporation of 7% arginine did not affect the physical and mechanical properties of the adhesive. Arginine was released from the adhesive at a rate and concentration that exhibited antibacterial effects, regardless of shifts in biofilm conditions such as sugar availability and pH.
Clinical significance: Secondary caries is recognized as the main reason for failure of dental restorations. The development of an arginine-based adhesive system has the potential to dramatically reduce the incidence and severity of secondary caries in adhesive restorations in a very economical fashion.

• 出版日期2017-8