Aptamer and Antisense-Mediated Two-Dimensional Isolation of Specific Cancer Cell Subpopulations

作者：Labib Mahmoud; Green Brenda; Mohamadi Reza M; Mepham Adam; Ahmed Sharif U; Mahmoudian Laili; Chang I Hsin; Sargent Edward H; Kelley Shana O^*

来源：Journal of the American Chemical Society, 2016, 138(8): 2476-2479.

DOI：10.1021/jacs.5b10939

Cancer cells, and in particular those found circulating in blood, can have widely varying phenotypes and molecular profiles despite a common origin. New methods are needed that can deconvolute the heterogeneity of cancer cells and sort small numbers of cells to aid in the characterization of cancer cell subpopulations: Here, we describe a new molecular approach to capturing cancer cells that isolates subpopulations using two-dimensional sorting. Using aptamer-mediated capture and antisense-triggered release, the new strategy sorts cells according to levels of two different markers and thereby sepatates them into their corresponding sub populations. Using a phenotypic assay) we demonstrate that the subpopulations isolated have markedly different properties. This system provides an important new tool for identifying circulating tumor cell subtypes.

• 出版日期2016-3-2