科研之友
微信
新浪微博
Facebook
分享链接
摘要
This paper describes a multi-layer paper-supported breast cancer tissue array. The paper. based microchip with arrayed hydrophilic spots was fabricated by wax printing. After cell seeding, the microchip was folded to form an artificial solid tumor, and the multilayer paper was covered by a layer of microporous membrane that mimics endothelial layer. After culturing the paper-supported breast cancer tissue for a certain period of time, the multi. layer device was unfold thus enabling detections of cell survival, proliferation, morphology and metabolism on different layers. Breast cancer cells cultured on paper had a survival rate > 80% and aggregated into tumor spheroids. Intracellular lactic acid levels increased with the extension of culture time. Compared to two dimensional (2D) counterparts, the decreased fraction of S phase cell (15% vs 60%) was detected. The paper. supported breast cancer tissue showed a drug response mechanism similar to the tumor in vivo. Cancer cells within the tissue were less sensitive to doxorubicin and the IC50 value detected was significantly higher than that obtained with 2D cultures (5 mu mol/L vs 1. 14 mu mol/L). The multi. layer paper-supported breast cancer tissue array developed in this work featured simple tissue reconstruction and easy operation, thus providing an ideal tool for anti. cancer drug testing.
