Adenosine Receptor Transcriptomic Profile in Cardiac Tissue of a Zucker Rat Model

作者：Cabiati Manuela; Svezia Benedetta; Guzzardi Maria Angela; Mattii Letizia; D'Amico Andrea; Caselli Chiara; Prescimone Tommaso; Morales Maria Aurora; Del Ry Silvia^*

来源：DNA and Cell Biology, 2015, 34(5): 333-341.

DOI：10.1089/dna.2014.2770

To evaluate the possible variations of adenosine receptor (AR) profile together with TNF-alpha and IL-6 mRNA in cardiac tissue of obese Zucker rats (OZR) during fasting conditions (fc) and during the induction of acute hyperglycemia (AH). OZR (O, n=21) and age-matched lean control rats (CO, n=18) were studied during fc (COfc, n=8; O-fc, n=13) and during the induction of AH (COAH, n=10; O-AH, n=8). The histopathologic analysis performed on O and CO heart samples did not show abnormalities of myocardial structure. The AR transcriptomic profile was analyzed in O and CO by real-time polymerase chain reaction (PCR) and a significantly lower mRNA expression was observed for A(2A)R in O with respect to CO (p=0.047), while a significant upregulation was observed for A(3)R in O with respect to CO (p=0.002). No significant differences between O and CO were observed for TNF-alpha or IL-6. Correlations were found between glycemia and A(1)R (p=0.03) and A(2B)R (p=0.002); total cholesterol and A(2B)R (p=0.02) and A(3)R (p=0.0002), as well as between IL-6 and A(1)R (p=0.05) and TNF-alpha and A(2A)R (p<0.0001). The modulation of ARs in these settings could represent a promising approach to pharmacological treatment, which must be supported by diet restrictions and physical exercise.

• 出版日期2015-5-1