Depletion of Ly6G/C+ cells ameliorates delayed cerebral vasospasm in subarachnoid hemorrhage

作者：Provencio J Javier^*; Altay Tamer; Smithason Saksith; Moore Shari Korday; Ransohoff Richard M

来源：Journal of Neuroimmunology, 2011, 232(1-2): 94-100.

DOI：10.1016/j.jneuroim.2010.10.016

• Neutrophils
• Subarachnoid hemmorhage
• Delayed cerebral vasospasm
• Inflammation
• Myeloid cells

Background: The etiology of delayed cerebral vasospasm (DCV) after aneurysmal subarachnoid hemorrhage (SAH) has remained elusive. Growing evidence supports a role for inflammation in the pathogenesis of DCV. We showed that CSF neutrophils predict which patients will develop DCV.
Methods: We evaluated a murine model of SAH to test the hypothesis that myeloid cells are required for the cerebral damage associated with DCV.
Results: SAN was associated with decreased middle cerebral artery caliber on day 1 which normalized at day 3 and recurred at day 6. In addition, behavioral testing with a Barnes maze showed executive dysfunction that progressively worsened after the seventh day post hemorrhage. To test the role of innate immune responses, we administrated a myeloid cell-depleting monoclonal antibody against Ly6G/C prior to experimental SAH. Myeloid cell depletion ameliorated angiographic vasospasm measured by MCA vessel caliber and normalized behavioral testing.
Conclusion: Our findings support the role of Ly6G/C+ cells in the development of DCV after SAN and suggest that immune modulation of neutrophils or other Ly6G/C+ cells may be a strategy for the prevention of DCV.

• 出版日期2011-3