Determination of Sterigmatocystin in Infant Cereals from Hangzhou, China

作者：Liu, Shaoying; Fan, Jicai; Huang, Xihui; Jin, Quan^*; Zhu, Guonian

来源：Journal of AOAC International, 2016, 99(5): 1273-1278.

DOI：10.5740/jaoacint.16-0098

• MYCOTOXINS
• MS/MS
• CHROMATOGRAPHY
• HPLC
• VALIDATION
• EXTRACTION
• ASPERGILLI
• CHEESE
• OCHRATOXIN
• RESIDUES

A simple method was developed for the determination of sterigmatocystin in infant cereals. The method consists of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction approach and cleanup with an SPE HLB cartridge. After purification for separation and quantification of sterigmatocystin, HPLC with UV detection and diode-array detection (DAD) was used at sterigmatocystin levels >10 mu g/kg, whereas ultra-performance LC with electrospray ionization (ESI) and tandem MS (MS/MS) was used at levels <10 mu g/kg. The compound was determined by UV-DAD at 325 nm and by ESI-MS/MS in the positive ionization mode. Good r(2) values (>= 0.99) were found with both UV-DAD and ESI-MS/MS, and satisfactory recoveries (84.8-96.2%) of sterigmatocystin in infant cereals were demonstrated, with RSD values 511.43%. The developed analytical method was used for the determination of sterigmatocystin in infant cereals from Hangzhou, China.

• 出版日期2016-9
• 单位杭州市疾病预防控制中心; 浙江大学