A SCHEME FOR SEQUENCING LARGE DNA-MOLECULES BY IDENTIFYING LOCAL NUCLEAR-INDUCED EFFECTS

作者：KELSON I^*; NUSSINOV S

来源：Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(15): 6963-6966.

DOI：10.1073/pnas.91.15.6963

An experimental scheme for sequencing large DNA molecules is proposed where DNA strands are replicated, with all nucleotides of a given kind marked with radioactive P-32. The marked strands are affixed to an appropriate substrate and are kept until most P-32 atoms decay. The local damage caused by the decay is expected to allow the identification of the sites occupied by that particular nucleotide, using atomic scale microscopy (scanning tunneling or atomic force microscopy). Quantitative aspects and methodological considerations associated with the proposed scheme are discussed.

• 出版日期1994-7-19