Advances in tracking hematopoiesis at the single-cell level

作者：Kokkaliaris Konstantinos D; Loeffler Dirk; Schroeder Timm^*

来源：Current Opinion in Hematology, 2012, 19(4): 243-249.

DOI：10.1097/MOH.0b013e32835421de

• cell tracking
• continuous single-cell imaging
• hematopoiesis
• stem cells

Purpose of review
Studying heterogeneous populations, such as hematopoietic stem cells (HSCs), requires continuous long-term observation of living cells at the single-cell level. The purpose of this review is to discuss recent advances in technologies required for continuous single-cell analysis and the contribution of this approach to find answers in hematopoiesis research.
Recent findings
Continuous long-term imaging at the single-cell level still requires custom-made hardware, software and manual in-depth analysis of large amounts of data. Despite these technical difficulties, continuous time-lapse imaging and single-cell tracking are increasingly used in hematopoiesis research. It has already contributed to answering decades-old questions.
Summary
Continuous long-term single-cell analysis is indispensable for a comprehensive analysis of dynamic processes in heterogeneous cell populations. Despite many remaining technological hurdles, this approach is increasingly used in hematopoiesis research.

• 出版日期2012-7